ice cold homogenization buffer (Thermo Fisher)

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Thermo Fisher ice cold homogenization buffer
Ice Cold Homogenization Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/homogenization+buffer/Triton+X-100/pmc13146351-67-7-44
Average 99 stars, based on 1 article reviews

ice cold homogenization buffer - by Bioz Stars, 2026-10

99/100 stars

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Homogenization:

Article Title: Adaptation of lipid metabolism of the polyextremophilic cyanobacterium Cyanobacterium aponinum PCC 10605 to adverse environmental conditions.
Article Snippet: .. E. coli cells were harvested and suspended in homogenization buffer (1 mM EDTA, 200 mM sucrose, 100 mM Tris-HCl, pH 7.4) and homogenized with a sonicator (Bioblock Scientific). ..

Article Title: Modeling cis -regulatory variation in human brain enhancers across a large Parkinson’s Disease cohort
Article Snippet: .. In brief, the tissue was homogenized and lysed in Homogenization Buffer (10 mM Tris-HCl (pH 7.5), 146 mM NaCl, 1 mM CaCl 2 , 21 mM MgCl 2 , 1% BSA (Gibco, 15260037), 25 mM KCl, 250 mM sucrose, 0.03% TWEEN® 20, 0.03% IGEPAL® CA-630 (Sigma-Aldrich, I8896), 1 mM dithiothreitol, 1× cOmplete protease inhibitor (Roche), and 1 U/μL RNasin® Plus Ribonuclease Inhibitor (Promega, N261B)). .. After lysis the nuclei were first filtered through a 70 μm EASYstrainer (Greiner, 542070), mixed with 1 mL of Wash Buffer 1 (WB1) (10 mM Tris-HCl (pH 7.5), 146 mM NaCl, 1 mM CaCl 2 , 21 mM MgCl 2 , 1% BSA, 25 mM KCl, 250 mM sucrose, 1 mM dithiothreitol, 1× cOmplete protease inhibitor (Roche), and 1 U/μL RNasin® Plus Ribonuclease Inhibitor (Promega)) and centrifuged (500 rcf) in a swinging bucket for 5 minutes at 4°C.

Article Title: The Legionella phosphocholinase AnkX modulates IMPDH2 regulation and cytoophidia dynamics
Article Snippet: .. At 1 h post-infection, the cells were collected by scraping, washed three times with ice-cold DPBS (Gibco), centrifuged (450 g , 5 min, 4°C), and resuspended in homogenization buffer (20 mM HEPES pH 7.2, 250 mM sucrose, 0.5 mM EGTA) containing protease inhibitors (Pierce Protease Inhibitor Mini Tablets, EDTA-free; Thermo Fisher). .. Subsequently, infected cells were passaged nine times through a ball homogenizer (Isobiotec) with an exclusion size of 8 μm.

Article Title: Adaptation of lipid metabolism of the polyextremophilic cyanobacterium Cyanobacterium aponinum PCC 10605 to adverse environmental conditions
Article Snippet: .. E. coli cells were harvested and suspended in homogenization buffer (1 mM EDTA, 200 mM sucrose, 100 mM Tris-HCl, pH 7.4) and homogenized with a sonicator (Bioblock Scientific). ..

Article Title: Molecular insights into the regulation of GNPTαβ by LYSET.
Article Snippet: .. The supernatant was discarded, and the pellet was resuspended in 3 ml of homogenization buffer (HB: 5 mM Tris, 250 mM Sucrose, 1 mM EGTA in mass-spectrometry grade water (ThermoFisher), pH 7.4. .. The supernatant was discarded, and the pellet was resuspended in 3 ml of homogenization buffer (HB: 5 mM Tris, 250 mM Sucrose, 1 mM EGTA in mass-spectrometry grade water (ThermoFisher), pH 7.4.

Article Title: An Unbiased Molecular Characterization of Peripartum Cardiomyopathy Hearts Identifies Mast Cell Chymase as a New Diagnostic Candidate
Article Snippet: .. Then they were homogenized using a 7 ml dounce homogenizer (Sigma-Aldrich) in homogenization buffer (250 mM sucrose, 25 mM KCl, 5 mM MgCl2, 10 mM Tris–HCl, 1 mM dithiothreitol (DTT), 1x protease inhibitor, 0.4 U/μl RNaseIn (Invitrogen), 0.2 U/μl SUPERase In (Invitrogen), 0.1% Triton X-100 in nuclease free water) with eight loose strokes with pestle A followed by eight tight strokes with pestle B. Homogenate was filtered through 40 μm cell strainer (pluriSelect) and centrifuged (500g, 5 min, 4 °C) to obtain nuclei pellet. .. The supernatant was removed and the nuclei pellet was resuspended in storage buffer (1x PBS -Mg/Ca), 1% bovine serum albumin, 0.2 U/μl Protector RNaseIn (Roche).

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Article Title: Molecular insights into the regulation of GNPTαβ by LYSET
Article Snippet: The Pearson coefficient analysis was performed using the FIJI ImageJ JACOP BIOP program.

Article Title: The Legionella phosphocholinase AnkX modulates IMPDH2 regulation and cytoophidia dynamics
Article Snippet: Imaging was performed using a Leica SP8 DMi8 CS with AFC laser scanning microscope, an HC PL APO CS2 63×/1.4 oil objective, and Leica LAS X software (Leica).

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